Focusing on the low accuracy problem of regular fingerprint matching indoor localization algorithm for Wi-Fi network confronted with signal fluctuation or jamming, the fingerprint matching indoor localization algorithm based on Dynamic Time Warping (DTW) similarity for Wi-Fi network was proposed. Firstly, the Wi-Fi signal characteristics in localization area were converted to the time-series fingerprints according to the sequence of sampling. The similarity between the locating data and sampling data was obtained by computing the fingerprint DTW distance of Wi-Fi signal. Then, according to the structural characteristics of the sampling area, the fingerprint sampling problem of Wi-Fi signal was divided into three kinds of basic sampling methods based on dynamic path. Finally, the accuracy and completeness of the fingerprint feature information were increased by the combination of multiple dynamic path sampling methods, which improved the accuracy and location precision of fingerprint matching. The extensive experimental results show that, compared with the instantaneous fingerprint matching indoor localization algorithm, within the location error of 3 m, the cumulative error frequency of the proposed localization algorithm, was 10% higher for uniform motion and 13% higher for variable motion within routing area, and 9% higher for crossed curvilinear motion and 3% higher for S-type curvilinear motion within open area. The proposed localization algorithm can improve accuracy and location precision of fingerprint matching effectively in real indoor localization applications.